Mouse Monoclonal to GST / Glutathione-S-Transferase, GST tag
S-tag-05 (IgG2b)
Technical Information Request Inquiry for Bulk Prices
|
Product No. |
Form |
Quantity |
Stock |
Price |
Datasheet |
Shop |
| |
11-477-C025 |
purified |
0.025 mg |
 |
choose region |
  |
 |
| |
11-477-C100 |
purified |
0.1 mg |
|
choose region |
|
|
- In stock! Ready for immediate despatch
- Manufacture on order
Background
The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Specificity
The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody
is suitable for detecting fusion proteins
containing the GST-Tag sequence expressed
in E. coli, yeast, mammalian, and
in vitro transcription/translation systems.
Immunogen
GST fusion protein
Species Reactivity:
- Recognizes fusion proteins in all species
Negative Species:
|
Applications:
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
|
References
Unpublished.
|
For research use only. Not for drug, diagnostic or other use.
Related Products
Mouse IgG2b Isotype Control
Example Data
Fig. 1. Isolation of GST
Fig. 1. Isolation of Glutathione-S-Transferase (GST) overexpressed in
E. coli.
Western blot was immunostained by anti-GST (
S-tag-05).
Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose
Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02; cat. no. 11-424-C100)
