Mouse Monoclonal to beta2-microglobulin
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non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
The antibody B2M-02 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Purified isolated human beta2-microglobulin.
- Flow Cytometry
Recommended dilution: 1 μg/ml
Positive control: PBL cell line
Negative control: DAUDI human lymphoma cell line, erythrocytes
- Western Blotting
Recommended dilution: 2-4 μg/ml
Positive control: U937 human lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Application note: Both reducing and non-reducing conditions.
- Immunohistochemistry (paraffin sections)
Recommended dilution: 5 μg/ml
Positive tissue: liver
Application note: Working dilution should be determinated by investigatore.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for detection and purification of
human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76. [Abstract]
*Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02