categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD9

MEM-61 (IgG1)

Product No.	Form	Quantity	Price
11-208-C025	purified	0.025 mg	choose region
11-208-C100	purified	0.1 mg	choose region
10-208-C025	azide free	0.025 mg	choose region
10-208-C100	azide free	0.1 mg	choose region
1B-208-C025	biotin	0.025 mg	choose region
1B-208-C100	biotin	0.1 mg	choose region
PB-208-T025	Pacific Blue™	25 tests	choose region
PB-208-T100	Pacific Blue™	100 tests	choose region
1F-208-T025	FITC	25 tests	choose region
1F-208-T100	FITC	100 tests	choose region
1A-208-T025	APC	25 tests	choose region
1A-208-T100	APC	100 tests	choose region
A6-208-T025	Alexa Fluor® 647	25 tests	choose region
A6-208-T100	Alexa Fluor® 647	100 tests	choose region

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Background

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Entrez Gene: 12p13.3

Specificity

The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
HLDA VI; WS Code P P-15

Immunogen

Pre-B cell line NALM-6.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution: 5 μg/ml Western Blotting Recommended dilution: 2-4 μg/ml Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 20 μg/ml Positive tissue: prostate Functional Application The antibody MEM-61 induces FcγR-dependent platelet aggregation. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65. [Abstract] [Full Text] Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7. [Abstract] Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455(2):283-96. [Abstract] Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9. [Abstract] [Full Text] Product Specific References Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009 Apr;20(7):2030-40. [Abstract] [Full Text] Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am J Physiol Cell Physiol. 2007 Nov;293(5):C1660-8. [Abstract] [Full Text] Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1;167(5):2724-33. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 5 μg/ml
Western Blotting
Recommended dilution: 2-4 μg/ml
Application note: Non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 20 μg/ml
Positive tissue: prostate
Functional Application
The antibody MEM-61 induces FcγR-dependent platelet aggregation.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65. [Abstract] [Full Text]

*Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7. [Abstract]

*Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455(2):283-96. [Abstract]

*Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9. [Abstract] [Full Text]

Product Specific References

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009 Apr;20(7):2030-40. [Abstract] [Full Text]

*Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am J Physiol Cell Physiol. 2007 Nov;293(5):C1660-8. [Abstract] [Full Text]

*Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1;167(5):2724-33. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9 (MEM-61) FITC. Total viable cells were used for analysis.

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