categories: CD and Related Antigens & MHC (Rodent)

Rat Monoclonal to CD8a (mouse)

53-6.7 (IgG2a)

Product No.	Form	Quantity	Price
10-579-C100	azide free	0.1 mg	choose region
PB-579-C025	Pacific Blue™	0.025 mg	choose region
PB-579-C100	Pacific Blue™	0.1 mg	choose region
1F-579-C100	FITC	0.1 mg	choose region
A4-579-C025	Alexa Fluor® 488	0.025 mg	choose region
A4-579-C100	Alexa Fluor® 488	0.1 mg	choose region
1P-579-C100	PE	0.1 mg	choose region
1A-579-C025	APC	0.025 mg	choose region
1A-579-C100	APC	0.1 mg	choose region
PC-579-C100	PerCP	0.1 mg	choose region
A7-579-C025	Alexa Fluor® 700	0.025 mg	choose region
A7-579-C100	Alexa Fluor® 700	0.1 mg	choose region

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- Manufacture on order

Background

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Entrez Gene: 6 30.5 cM

Specificity

The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa; alpha chain of the CD8 antigen).

Immunogen

Mouse spleen cells

Species Reactivity: Mouse Negative Species:	Applications: Flow Cytometry Recommended dilution:1.5 μg/ml Immunoprecipitation Immunohistochemistry (paraffin sections) Immunohistochemistry (frozen sections) Recommended dilution: 1:1000 Application note: Formaldehyde fixation is not recommended, acetone fixation is preferred. Immunocytochemistry Functional Application Isolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cell proliferation. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. [Abstract] Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95. [Abstract] [Full Text] Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5557-61. [Abstract] [Full Text] Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug;114(4):582-8. [Abstract] [Full Text] Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan;109(2):183-92. [Abstract] [Full Text] Ko SY, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175(5):3309-17. [Abstract] [Full Text] Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1;177(1):306-14. [Abstract] [Full Text] Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5102-7. [Abstract] [Full Text] Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. [Abstract] [Full Text] And many other.

Species Reactivity:

Mouse

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1.5 μg/ml
Immunoprecipitation
Immunohistochemistry (paraffin sections)
Immunohistochemistry (frozen sections)
Recommended dilution: 1:1000
Application note: Formaldehyde fixation is not recommended, acetone fixation is preferred.
Immunocytochemistry
Functional Application
Isolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cell proliferation.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. [Abstract]

*Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95. [Abstract] [Full Text]

*Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5557-61. [Abstract] [Full Text]

*Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug;114(4):582-8. [Abstract] [Full Text]

*Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan;109(2):183-92. [Abstract] [Full Text]

*Ko SY, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175(5):3309-17. [Abstract] [Full Text]

*Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1;177(1):306-14. [Abstract] [Full Text]

*Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5102-7. [Abstract] [Full Text]

*Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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