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Mouse Monoclonal to ARHGEF4 / ASEF1

ARHGEF-08 (IgG1)

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Background

ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.

Specificity

The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42.

Regulatory Status

Immunogen

Recombinant fragment of human ARHGEF4 (amino acids 143-271)

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Application note:intracellular staining
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase. J Biol Chem. 2009 Aug 14;284(33):22436-43. [Abstract]
  • *Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation. Leuk Res. 2010 Jan;34(1):109-15. [Abstract]
  • *Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell Res. 2012 Feb;22(2):372-86. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    ARHGEF4 WB
    Fig. 1.


    Fig. 1. Western blotting analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfectants (B) using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08).

    ARHGEF PE


    Fig. 2. Intracellular staining of ARHGEF4 in transfected HEK-293 cells using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08) PE.

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