Mouse Monoclonal to beta-Catenin

EM-22 (IgG1)

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-557-C025	purified	0.025 mg		choose region
	11-557-C100	purified	0.1 mg		choose region

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Background

Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.

Entrez Gene: 3p21

Specificity

The antibody EM-22 reacts with C-terminal part of beta-catenin, an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.

Immunogen

Recombinant human beta-catenin

Species Reactivity: Human Mouse Hamster Negative Species:	Applications: Flow Cytometry Immunoprecipitation Western Blotting Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line KW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line Immunocytochemistry Positive control: HT29 human colon adenocarcinoma cell line Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Nowak M, Madej JA, Dziegiel P.: Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia Histochem Cytobiol. 2007;45(3):233-8. [Abstract] Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS.: Epstein-Barr Virus, Beta-Catenin, and E-cadherin in Gastric Carcinomas. J Korean Med Sci. 2007 Oct;22(5):855-61. [Abstract] *Valenta T, Lukas J, Korinek V.: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31(9):2369-80. [Abstract] [Full Text]

Species Reactivity:

Human
Mouse
Hamster

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Western Blotting
Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line
KW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line
Immunocytochemistry
Positive control: HT29 human colon adenocarcinoma cell line

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Nowak M, Madej JA, Dziegiel P.: Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia Histochem Cytobiol. 2007;45(3):233-8. [Abstract]

*Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS.: Epstein-Barr Virus, Beta-Catenin, and E-cadherin in Gastric Carcinomas. J Korean Med Sci. 2007 Oct;22(5):855-61. [Abstract]

*Valenta T, Lukas J, Korinek V.: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31(9):2369-80. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green). Cell nuclei visualized by DAPI (blue).

Fig. 2.

Fig. 2. Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody.

Fig. 3.

Fig. 3. Flow cytometry analysis (intracellular) of beta-catenin in human MCF-7 cell line by EM-22-Alexa Fluor® 488 (red) compared to isotype control (blue).

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