Mouse Monoclonal to CD52 / CAMPATH-1
HI186 (IgG2b)
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Background
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
Specificity
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
HLDA VI; WS Code BP 523
HLDA VI; WS Code T 6T-057
Immunogen
Human tonsil
Species Reactivity:
Negative Species:
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Applications:
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
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General references
*Treumann A, Lifely MR, Schneider P, Ferguson MA: Primary structure of CD52. J Biol Chem. 1995 Mar 17;270(11):6088-99.
[Abstract] [Full Text]
*Rowan WC, Hale G, Tite JP, Brett SJ: Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan;7(1):69-77.
[Abstract]
*Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C: Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15;274(42):29862-73.
[Abstract] [Full Text]
*Domaga³a A, Kurpisz M: CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr;7(2):325-31.
[Abstract]
*Koyama K, Ito K, Hasegawa A: Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 2007;63:103-10.
[Abstract]
Product Specific References
*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
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For research use only. Not for drug, diagnostic or other use.
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Mouse IgG2b Isotype Control
Example Data
Fig. 1A Flow Cytometry analysis
Fig. 1. Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE).
Fig. 1A – CD52 positive cALL (gate leukemic cells)
Fig. 1B – CD52 negative pro T ALL (gate leukemic cells)
Fig. 1B Flow Cytometry analysis
