categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD44 / Pgp-1

MEM-85 (IgG2b)

Product No.	Form	Quantity	Price
11-221-C025	purified	0.025 mg	choose region
11-221-C100	purified	0.1 mg	choose region
1B-221-C025	biotin	0.025 mg	choose region
1B-221-C100	biotin	0.1 mg	choose region
PB-221-T025	Pacific Blue™	25 tests	choose region
PB-221-T100	Pacific Blue™	100 tests	choose region
1F-221-T025	FITC	25 tests	choose region
1F-221-T100	FITC	100 tests	choose region
A4-221-T025	Alexa Fluor® 488	25 tests	choose region
A4-221-T100	Alexa Fluor® 488	100 tests	choose region
1P-221-T025	PE	25 tests	choose region
1P-221-T100	PE	100 tests	choose region
1A-221-T025	APC	25 tests	choose region
1A-221-T100	APC	100 tests	choose region
A6-221-T025	Alexa Fluor® 647	25 tests	choose region
A6-221-T100	Alexa Fluor® 647	100 tests	choose region

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Background

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Entrez Gene: 11p13

Specificity

The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
HLDA IV; WS Code NL 706
HLDA VI; WS Code AS Ref.15

Immunogen

Leukocytes of a patient suffering from LGL Type Leukaemia.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:1 μg/ml Immunoprecipitation Western Blotting Recommended dilution: 2 μg/ml, 60 min on vertical incubator Positive control: Kg-1a human leukemia cell lysate Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). ELISA Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12 [Abstract] [Full Text] Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug;37(2):248-53. [Abstract] Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb;4(1):59-63. [Abstract] Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec;83(3):341-6. [Abstract] Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp Mol Pathol. 2007 Oct;83(2):207-15. [Abstract] Product Specific References Stefanová I, Hilgert I, Bazil V, Kristofová H, Horejsí V: Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules. Immunogenetics. 1989;29(6):402-4. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Bazil V, Strominger JL: Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes. Induction of cleavage of L-selectin via CD16. J Immunol. 1994 Feb 1;152(3):1314-22. [Abstract] Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8. [Abstract] [Full Text] Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109(6):2545-52. [Abstract] [Full Text] Schmidt D, Achermann J, Odermatt B, Breymann C, Mol A, Genoni M, Zund G, Hoerstrup SP: Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source. Circulation. 2007 Sep 11;116(11 Suppl):I64-70. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1 μg/ml
Immunoprecipitation
Western Blotting
Recommended dilution: 2 μg/ml, 60 min on vertical incubator
Positive control: Kg-1a human leukemia cell lysate
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
ELISA

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12 [Abstract] [Full Text]

*Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug;37(2):248-53. [Abstract]

*Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb;4(1):59-63. [Abstract]

*Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec;83(3):341-6. [Abstract]

*Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp Mol Pathol. 2007 Oct;83(2):207-15. [Abstract]

Product Specific References

*Stefanová I, Hilgert I, Bazil V, Kristofová H, Horejsí V: Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules. Immunogenetics. 1989;29(6):402-4.

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Bazil V, Strominger JL: Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes. Induction of cleavage of L-selectin via CD16. J Immunol. 1994 Feb 1;152(3):1314-22. [Abstract]

*Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8. [Abstract] [Full Text]

*Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109(6):2545-52. [Abstract] [Full Text]

*Schmidt D, Achermann J, Odermatt B, Breymann C, Mol A, Genoni M, Zund G, Hoerstrup SP: Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source. Circulation. 2007 Sep 11;116(11 Suppl):I64-70. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG2b Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of human peripheral blood cells with anti-human CD44 (MEM-85) PE.

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