Mouse Monoclonal to CD222 / IGF2 receptor / CIMPR
MEM-238 (IgG1)
Technical Information Request Inquiry for Bulk Prices
- In stock! Ready for immediate despatch
- Manufacture on order
Background
CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
Entrez Gene (human):
6q26
Specificity
The antibody MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
Immunogen
Recombinant Vaccinia virus encoding CD222.
Species Reactivity:
Negative Species:
|
Applications:
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
|
General references
*Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
*Gasanov U, Koina C, Beagley KW, Aitken RJ, Hansbro PM: Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes. Infect Immun. 2006 Jan;74(1):566-77.
[Abstract] [Full Text]
*Wood RJ, Hulett MD: Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222)
binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. J Biol Chem. 2008 Feb 15;283(7):4165-76.
[Abstract]
Product Specific References
*Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H: The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem. 2002 Oct 25;277(43):40575-82.
[Abstract] [Full Text]
|
For research use only. Not for drug, diagnostic or other use.
Related Products
Mouse IgG1 Isotype Control
Example Data
Fig. 1. Western Blotting
Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line.
Lane 1: immunostaining with anti-CD222 (
MEM-238)
Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)
