categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD205

HD30 (IgG1)

Product No.	Form	Quantity	Price
11-669-C025	purified	0.025 mg	choose region
11-669-C100	purified	0.1 mg	choose region
1P-669-T025	PE	25 tests	choose region
1P-669-T100	PE	100 tests	choose region

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Background

CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.

Entrez Gene: 2q24

Specificity

The mouse monoclonal antibody HD30 recognizes CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

Immunogen

Recombinant Fc-tagged human CD205

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Western Blotting Application note:only non-denatured samples Immunohistochemistry (frozen sections) Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008 Aug 15;112(4):1231-9. [Abstract] [Full Text] Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 2000 Aug;61(8):729-38. [Abstract] Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments. J Cell Biol. 2000 Oct 30;151(3):673-84. [Abstract] [Full Text] Product Specific References Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. 2012 Mar 30;377(1-2):15-22. [Abstract]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Western Blotting
Application note:only non-denatured samples
Immunohistochemistry (frozen sections)

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008 Aug 15;112(4):1231-9. [Abstract] [Full Text]

*Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 2000 Aug;61(8):729-38. [Abstract]

*Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments. J Cell Biol. 2000 Oct 30;151(3):673-84. [Abstract] [Full Text]

Product Specific References

*Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. 2012 Mar 30;377(1-2):15-22. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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