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Mouse Monoclonal to ABRA1

ABRA1-01 (IgG)

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Background

ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.

Specificity

The mouse monoclonal antibody ABRA1-01 recognizes N-terminal part of ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.

Regulatory Status

Immunogen

Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag

Species Reactivity:

  • Human
  • Other not tested

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1-2 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Application note: reducing conditions
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007 May 25;316(5828):1194-8. [Abstract]
  • *Liu Z, Wu J, Yu X: CCDC98 targets BRCA1 to DNA damage sites. Nat Struct Mol Biol. 2007 Aug;14(8):716-20. [Abstract]
  • *Wang B, Elledge SJ: Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20759-63. [Abstract] [Full Text]
  • *Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA: Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene. 2009 Apr 23;28(16):1843-52. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    ABRA1
    Fig. 1.


    Fig. 1. Detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells.
    Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01)
    Lane B: Immunostaining with anti-EGFP (polyclonal)
    Lane C: Immunostaining with anti-alpha tubulin (clone TU-01)

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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