categories: Adaptor Proteins (Human), Transcription Regulation & Nuclear Proteins (Human)

Mouse Monoclonal to ABRA1

ABRA1-01 (IgG)

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-619-C025	purified	0.025 mg		choose region
	11-619-C100	purified	0.1 mg		choose region

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Background

ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.

Entrez Gene: 4q21.23

Specificity

The mouse monoclonal antibody ABRA1-01 recognizes N-terminal part of ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.

Immunogen

Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag

Species Reactivity: Human Other not tested Negative Species:	Applications: Flow Cytometry Recommended dilution:1-2 μg/ml Immunoprecipitation Western Blotting Application note: reducing conditions Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007 May 25;316(5828):1194-8. [Abstract] Liu Z, Wu J, Yu X: CCDC98 targets BRCA1 to DNA damage sites. Nat Struct Mol Biol. 2007 Aug;14(8):716-20. [Abstract] Wang B, Elledge SJ: Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20759-63. [Abstract] [Full Text] Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA: Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene. 2009 Apr 23;28(16):1843-52. [Abstract] [Full Text]

Species Reactivity:

Human
Other not tested

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1-2 μg/ml
Immunoprecipitation
Western Blotting
Application note: reducing conditions

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007 May 25;316(5828):1194-8. [Abstract]

*Liu Z, Wu J, Yu X: CCDC98 targets BRCA1 to DNA damage sites. Nat Struct Mol Biol. 2007 Aug;14(8):716-20. [Abstract]

*Wang B, Elledge SJ: Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20759-63. [Abstract] [Full Text]

*Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA: Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene. 2009 Apr 23;28(16):1843-52. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Example Data

Fig. 1.

Fig. 1. Detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells.
Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01)
Lane B: Immunostaining with anti-EGFP (polyclonal)
Lane C: Immunostaining with anti-alpha tubulin (clone TU-01)

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