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Assay Kit

BSA - FITC

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EXB0013 5 mg yes choose region View datasheet buy
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Description

Bovine serum albumin (BSA) is a 66 kDa globular protein abundant in the blood. Albumins contain high affinity binding sites for many cellular metabolites such as long chain fatty acids, vitamins, hormones and metal ions and deliver them to the cells by passage from the capillary lumen through the endothelium into the intersticial space. Fluorescence conjugates of albumin are used not only to reveal the physiology of albumin within the tissues, but above all as probes to study various types of endocytosis.

The reagent is intended for investigation of endocytosis and endocytic recycling pathways in mammals.

Regulatory Status

Format/Formulation

Lyophilized

General references

  • *Sabah JR, Davidson H, McConkey EN, Takemoto L: In vivo passage of albumin from the aqueous humor into the lens. Mol Vis. 2004 Apr 2;10:254-9. [Abstract] [Full Text]
  • *Chu Y, Piper R, Richardson S, Watanabe Y, Patel P, Heistad DD: Endocytosis of extracellular superoxide dismutase into endothelial cells: role of the heparin-binding domain. Arterioscler Thromb Vasc Biol. 2006 Sep;26(9):1985-90. [Abstract] [Full Text]
  • *Poteryaev D, Fares H, Bowerman B, Spang A: Caenorhabditis elegans SAND-1 is essential for RAB-7 function in endosomal traffic. EMBO J. 2007 Jan 24;26(2):301-12. [Abstract]
  • *Yang YW, Shen SS: Enhanced antigen delivery via cell death induced by the vaccine adjuvants. Vaccine. 2007 Nov 7;25(45):7763-72. [Abstract]
  • *Sabah JR, Schultz BD, Brown ZW, Nguyen AT, Reddan J, Takemoto LJ: Transcytotic passage of albumin through lens epithelial cells. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1237-44. [Abstract]
  • Related Products

  • BSA - Dyomics 547
  • Example Data


    BSA-FITC
    Fig. 1. Immunofluorescence staining (HeLa cells)


    Fig. 1. Immunofluorescence staining of HeLa human cervix carcinoma cell line with Bovine Serum Albumin-FITC (20 μg/ml, 120 min in serum-free medium, 37°C; green).
    The BSA protein (green) is localizated in lysosomes/late endosomes. Nuclei are stained with DAPI (blue). The incubation in serum-free medium is recommended.

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