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Assay Kit

Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC

Product No. Quantity Stock Price Datasheet Shop
6K01-T050 50 tests yes choose region View datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Description

The Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC consists of the mixture of antibodies which stain human lymphocytes, monocytes, eosinophils and neutrophils. Peripheral blood dendritic cells and basophils can be identified by lack of staining with this reagent.

Regulatory Status

Format/Formulation

The reagent contains premixed combination of mouse monoclonal antibodies against human CD3, CD14, CD16, CD19, CD20 and CD56 antigens (clone MEM 57, MEM-15, LNK16, LT19, LT20 and MEM-188, respectively) labeled with Fluorescein isothiocyanate (FITC). Labeled antibodies are diluted in an optimal concentration in phosphate buffered saline (PBS) containing 15mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. The content of a vial (1 ml) is sufficient for 50 tests.

Sample size

20 μl of the Lineage Cocktail / 100 μl of blood

Product Specific References

  • For antibody specific references see the datasheet.
  • Example Data

    dend
    Fig. 1.

    Fig. 1. Identification of dendritic cells in human peripheral blood by their lack of staining with the Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC and positive staining with anti-HLA-DR antibody conjugated with PE. 100 000 events were acquired for identification of HLA-DR+ dendritic cells.

    bas
    Fig. 2.

    Fig. 2. Identification of basophils in human peripheral blood by their lack of staining with the Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC and positive staining with anti-IgE antibody conjugated with PE-Dyomics 647. 60 000 events were acquired for identification of IgE+ basophils.

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