Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC
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The Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC consists of the mixture of antibodies which stain human lymphocytes, monocytes, eosinophils and neutrophils. Peripheral blood dendritic cells and basophils can be identified by lack of staining with this reagent.
The reagent contains premixed combination of mouse monoclonal antibodies against human CD3, CD14, CD16, CD19, CD20 and CD56 antigens (clone MEM 57, MEM-15, LNK16, LT19, LT20 and MEM-188, respectively) labeled with Fluorescein isothiocyanate (FITC). Labeled antibodies are diluted in an optimal concentration in phosphate buffered saline (PBS) containing 15mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. The content of a vial (1 ml) is sufficient for 50 tests.
20 μl of the Lineage Cocktail / 100 μl of blood
Product Specific References
For antibody specific references see the datasheet.
Fig. 1. Identification of dendritic cells in human peripheral blood by their lack of staining with the Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC and positive staining with anti-HLA-DR antibody conjugated with PE. 100 000 events were acquired for identification of HLA-DR+ dendritic cells.
Fig. 2. Identification of basophils in human peripheral blood by their lack of staining with the Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC and positive staining with anti-IgE antibody conjugated with PE-Dyomics 647. 60 000 events were acquired for identification of IgE+ basophils.