Assay Kit
Human Transferrin - Dyomics 547
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NL-009-M005 |
5 mg |
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Description
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH.
Internalization of labeled transferrin thus represents an usefull approach to study endocytosis.
The reagent is intended for investigation of endocytosis, endocytic recycling pathways and
transferrin receptor binding affinity in mammals.
Format/Formulation
Lyophilized
General references
*Hansen SH, Sandvig K, van Deurs B: Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification. J Cell Biol. 1993 Apr;121(1):61-72.
[Abstract] [Full Text]
*Ghosh RN, Gelman DL, Maxfield FR: Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy. J Cell Sci. 1994 Aug;107 ( Pt 8):2177-89.
[Abstract] [Full Text]
*Ghosh RN, Maxfield FR: Evidence for nonvectorial, retrograde transferrin trafficking in the early
endosomes of HEp2 cells. J Cell Biol. 1995 Feb;128(4):549-61.
[Abstract] [Full Text]
*Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79
[Abstract] [Full Text]
*Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318.
[Abstract] [Full Text]
*Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36. [Abstract] [Full Text]
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Human Transferrin - FITC
Example Data
Fig. 1. Immunofluorescence staining (human skin fibroblasts)
Fig. 1. Immunofluorescence staining of human skin fibroblasts with
Human Transferrin – Dyomics 547 (5 μg/ml, 30 min, 37°C; red).
Co-localization with lysosomal/endosomal/granule protein shown by CD63 antigen staining with purified anti-human CD63 (MEM-259; cat. no. 11-343-C100) (detection by Goat anti-mouse IgG1 Alexa Fluor® 488; green). Nuclei were stained with DAPI (blue).
Fig. 2. Immunofluorescence staining (human macrophages)
Fig. 2. Immunofluorescence staining of human macrophages (derived from the peripheral blood monocytes) with
Human Transferrin – Dyomics 547 (red).
Co-localization
with MHC Class II molecules (green) shown by staining with purified anti-human HLA-DR+DP (MEM-136; cat. no. 11-296-C100) (detection by Goat anti-mouse IgG1 Alexa Fluor® 488; green). Nuclei were
stained with DAPI (blue).
(Please, enlarge the figures and see the details.)
