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Assay Kit


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RD194070100R 96 tests yes choose region View datasheet buy
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sHLA-G ELISA kit is suitable for the determination of soluble forms of Human Leukocyte Antigen-G (sHLA-G) in amniotic fluid, cell culture supernatant, plasma and serum.
In this sHLA-G ELISA, standards and samples are incubated in microtitration wells coated with mouse monoclonal antibody MEM-G/9, which recognizes the most abundant soluble isoforms, shedded HLA-G1 and intron4-containing secreted HLA-G5.
Immobilized antibody/sHLA-G complex is detected by mouse monoclonal anti human beta2-microglobulin antibody conjugated with horseradish peroxidase (HRP).

Regulatory Status


colorimetric double monoclonal sandwich ELISA (HRP-conjugated detection antibody)

Sample size

50 - 100 μl

Incubation time

< 4 hours

Product Specific References

  • *Luque J, Torres MI, Aumente MD, Marin J, Garcia-Jurado G, Gonzalez R, Pascual D, Guerra N, Lopez-Rubio F, Alvarez-Lopez MR, Arizon JM, Pena J: Soluble HLA-G in heart transplantation: their relationship to rejection episodes and immunosuppressive therapy. Hum Immunol. 2006 Apr-May;67(4-5):257-63. [Abstract]
  • *Torres MI, Lopez-Casado MA, Luque J, Pena J, Rios A: New advances in coeliac disease: serum and intestinal expression of HLA-G. Int Immunol. 2006 May;18(5):713-8. [Abstract]
  • *Desai N, Filipovits J, Goldfarb J: Secretion of soluble HLA-G by day 3 human embryos associated with higher pregnancy and implantation rates: assay of culture media using a new ELISA kit. Reprod Biomed Online. 2006 Aug;13(2):272-7. [Abstract]
  • *White SR, Loisel DA, McConville JF, Stern R, Tu Y, Marroquin BA, Noth I, Ober C: Levels of soluble human leukocyte antigen-G are increased in asthmatic airways. Eur Respir J. 2010 Apr;35(4):925-7. [Abstract] [Full Text]
  • *Basturk B, Karakayali F, Emiroglu R, Sozer O, Haberal A, Bal D, Haberal M: Human leukocyte antigen-G, a new parameter in the follow-up of liver transplantation. Transplant Proc. 2006 Mar;38(2):571-4. [Abstract]
  • Example Data

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