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The TregFlowEx® Kit is designed for the detection of regulatory T cells (CD4+ CD25+ FOXP3+ cells) in human peripheral blood or human umbilical cord blood using flow cytometry.
TregFlowEx® Kit contains following reagents:
Fix and Lysing Solution - 1 vial containing 10 ml of 10x concentrated reagent.
Permeabilizing Solution - 1 vial containing 25 ml of reagent, ready to use.
Blocking Buffer - 1 vial containing 2.5 ml of reagent, ready to use.
CD4 FITC / CD25 PE - 1 vial containing 0.5 ml of cell surface staining reagent, a mixture of mouse monoclonal antibody against CD4, FITC labelled (clone MEM-241, isotype IgG1) and antibody against CD25, R-PE labeled (clone MEM-181, isotype IgG1).
FOXP3 APC - 1 vial containing 0.25 ml of monoclonal antibody against FOXP3, APC labelled (clone 3G3, isotype IgG1).