BrdUFlowEx® FITC Kit
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The BrdUFlowEx® FITC Kit is intended for labeling and subsequent immunodetection of proliferating cells by flow
cytometry. The kit can be used to analyze proliferation of
human or mouse leukocytes and variety of human and
murine cell lines.
The BrdUFlowEx® FITC Kit utilizes unique DNA revealing method known as "atomic scissors". The kit can be used not only for identification of proliferating cells, but also for simultaneous detection of nuclear proteins including those which are usually lost during other DNA revealing methods.
The BrdUFlowEx® FITC Kit is compatible with EdU staining, as the anti-BrdU antibody (clone MoBu-1) does not crossreact with EdU.
BrdUFlowEx® FITC Kit contains following reagents:
Fix and Lysing Solution: 1 vial containing 10x concentrated fixation and
erythrocyte lysing solution, the vial is intended for 100 reactions.
Permeabilizing Solution: 1 vial containing 30 ml of ready-to-use cellular
membrane permeabilizing solution, the vial is intended for 100 reactions.
AS Solution 1: (the abbreviation AS stands for atomic scissors), 4 vials
containing lyophilized sodium ascorbate, 1 vial is intended for 25
AS Solution 2: (the abbreviation AS stands for atomic scissors), 1 vial
containing 10 ml of ready-to-use copper (II) solution, the vial is
intended for 100 reactions.
AS Solution 3: (the abbreviation AS stands for atomic scissors), 2 vials
containing 30 ml of ready-to-use buffer, one vial is intended for 50
Anti-BrdU FITC: 1 vial containing 1 ml of ready-to-use solution
containing FITC labeled anti-BrdU antibody (clone MoBu-1, IgG1), the vial
is intended for 100 reactions.
7-AAD: 1 vial containing 2 ml of ready-to-use 7-aminoactinomycin D
solution, the vial is intended for 100 reactions.
BrdU: 4 vials containing lyophilized 5-bromo-2-deoxyuridine, 1 vial is
intended to prepare 2 ml of 10 mM BrdU solution.