KOMBITEST™ CD8 FITC / CD4 PE / CD3 PE-DY647
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The KOMBITEST™ CD8 FITC / CD4 PE / CD3 PE-DY647 is
designed for percentage enumeration of mature human
helper/inducer (CD3+CD4+) and suppressor/cytotoxic
(CD3+CD8+) T lymphocytes in erythrocyte-lysed whole blood.
The helper/suppressor ratio (CD4+ / CD8+) may also be
determined. This test is based on specific binding of monoclonal antibodies
to the antigenic determinants expressed on the surface of
leukocytes. The monoclonal antibodies are labeled with
different fluorochromes which are excited via single laser beam
from a flow cytometer during analysis. Subsequent emissions of
light from fluorochromes of each cell are collected and analyzed
by a flow cytometer. The fluorescence intensity differences
enable separation of cell subsets based on expression of
Regulatory status: RUO
Product Specific References
Anti-CD3, clone MEM-57: HLDA IV.; WS Code T 96
Anti-CD4, clone MEM-241: HLDA VIII; WS Code M241
Anti-CD8, clone MEM-31: HLDA III; WS Code T 575
Fig. 1. Lymphocytes in a dot-plot CD8 FITC vs. CD3 PE-Dyomics 647.
Fig. 2. Lymphocytes in a dot-plot CD4 PE vs. CD3 PE-Dyomics 647.