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Diagnostic Product

FagoFlowEx® Kit

Product No. Quantity Stock Price Datasheet Shop
ED7042 100 tests yes choose region View datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Description

The FagoFlowEx® Kit is intended for examination of phagocytic activity of granulocytes by measuring the respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow cytometry.

Regulatory Status

Format/Formulation

FagoFlowEx® Kit contains following reagents:

1. ED7042-1
E.coli – 5 vials containing lyophilized E. coli bacteria, 1 vial is intended for stimulation of 20 tubes.

2. ED7042-2
DHR123 – 5 vials containing lyophilized dihydrorhodamine 123, 1 vial is intended for staining of 60 tubes.

3. ED7042-3
PMA – 5 vials containing lyophilized PMA (phorbol 12-myristate 13-acetate), 1 vial is intended for stimulation of 20 positive controls.

4. ED7042-4
Lysing Solution – 15 ml

General references

  • Heyworth P, Cross A, Curnutte J (2003) Chronic granulomatous disease. Curr Opin Immunol 15(5): 578-84

    Winkelstein J, Marino M, Johnston R et al (2000) Chronic granulomatous disease. Report on a national registry of 368 patients. Medicine (Baltimore) 79 (3):155-69

    Sawyer DW, Donowitz GR, Mandell GL (1989) Polymorphonuclear neutrophils: An effective antimicrobial force. Rev Infect Dis 11: 1532-44
  • Product Specific References

  • *Porpodis K, Domvri K, Zarogoulidis P, Petridis D, Tsirgogianni K, Papaioannou A, Hatzizisi O, Kioumis I, Liaka A, Kikidaki V, Lampaki S, Organtzis J, Zarogoulidis K: Roflumilast, a phosphodiesterase-4 inhibitor, induces phagocytic activity in Greek COPD patients. Int J Chron Obstruct Pulmon Dis. 2015 Jun 15;10:1123-8.
  • *Hromádková L, Zárybnická L, Vávrová J, Bílková Z, Šinkorová Z: The impact of pre-analytical treatment and sorting on human neutrophil function. Veterinární Medicína, 59, 2014 (11): 543-552
  • *Jbeily N, Suckert I, Gonnert FA, Acht B, Bockmeyer CL, Grossmann SD, Blaess MF, Lueth A, Deigner HP, Bauer M, Claus RA: Hyperresponsiveness of mice deficient in plasma-secreted sphingomyelinase reveals its pivotal role in early phase of host response. J Lipid Res. 2013 Feb;54(2):410-24. [Abstract] [Full Text]
  • *Jeraiby M, Sidi Yahya K, Depince-Berger AE, Lambert C: Microbicidal activity measured by flow cytometry: Optimization and standardization for detection of primary and functional deficiencies. J Immunol Methods. 2016 Sep 29. pii: S0022-1759(16)30220-4. [Abstract]
  • Example Data


    Fago pacienti
    Fig. 1.


    Fig. 1. Examples of data obtained with FagoFlowEx Kit.
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