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The BasoFlowEx® Kit is intended for flow cytometry
examination of IgE-mediated allergic reactions via analysis
of CD63 antigen exposition on basophils in human heparinized
whole blood upon allergen stimulation.
BasoFlowEx® Kit contains following reagents:
Stimulation Buffer – 5 lyophilized vials, 1 vial
is intended for stimulation of 20 tubes.
Stimulation Control – 2 lyophilized vials, 1
vial is intended for stimulation of 25 positive controls.
Staining Reagent – 1 vial containing 2 ml of
premixed antibody cocktail: anti-CD63, FITC labeled +
anti-CD203c, PE labeled.
Lysing Solution – 30 ml.
Product Specific References
*The anti-CD203c antibody was described during HLDA VIII workshop.
The anti-CD63 antibody MEM-259 was described in: Cerny J et al., EMBO Rep. 2004 Sep;5(9):883-8.
*Rentzos G, Lundberg V, Lundqvist C, Rodrigues R, van Odijk J, Lundell AC, Pullerits T, Telemo E: Use of a basophil activation test as a complementary diagnostic tool in the diagnosis of severe peanut allergy in adults. Clin Transl Allergy. 2015 Jun 11;5:22. doi: 10.1186/s13601-015-0064-9. [Abstract] [Full Text]
*Depince-Berger AE, Sidi-Yahya K, Jeraiby M, Lambert C: Basophil activation test: Implementation and standardization between systems and between instruments. Cytometry A. 2017 Mar;91(3):261-269. [Abstract]
Fig. 1. Delimitation of basophil population (CD203cpos/ SSClow).
Fig. 2a Histogram of negative control basophils.
Fig. 2b Histogram of allergen-stimulated basophils.
Fig. 2c Histogram of positive control basophils.