EXCELLYSE I 100 ml
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Lysing solution 100 ml (500-1000 tests)
The EXCELLYSE I 100 ml lysing solution is intended for red blood cell lysis following antibody staining of human peripheral blood leukocytes. The lysing solution is appropriate for use with EXBIO single colour monoclonal antibodies and KOMBITEST™ reagents and can be used in both lyse/wash and lyse/no wash protocol.
Features for lyse/no wash protocol:
2-step lysing procedure
Excellent separation of lymphocytes and debris in SSC vs. FSC dot plot
EXCELLYSE I lysing solution is a ready to use reagent
provided as 100 ml package of proprietary buffered solution containing < 5 % formaldehyde. This quantity is sufficient for 500-1000 tests.
Usage and storage
Store the EXCELLYSE I lysing solution at room temperature.
P. H. Carter, S. Resto-Ruiz, G. C. Washington, S. Ethridge, A. Palini, R. Vogt, M. Waxdal, T. Fleisher, P. D. Noguchi, G. E. Marti: Flow cytometric analysis of whole blood lysis, three anticoagulants, and five cell preparations, Cytometry 13:68-74 (1992). Wiley-Liss, Inc. 1992.
K. R. Tamul, J. L. Schmitz, K. Kane, and J. D. Folds: Comparison of the effects of Ficoll-Hypaque separation and whole blood lysis on results of immunophenotypic analysis of blood and bone marrow samples from patients with hematologic malignancies, Clin. Diagn. Lab. Immunol. 1995 May; 2(3): 337–342.