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The FagoFlowEx® Kit is intended for examination of
phagocytic activity of granulocytes by measuring the
respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow
Regulatory status: RUO
FagoFlowEx® Kit contains following reagents:
E.coli – 5 vials containing lyophilized E. coli
bacteria, 1 vial is intended for stimulation of 20 tubes.
DHR123 – 5 vials containing lyophilized
dihydrorhodamine 123, 1 vial is intended for staining of 60
PMA – 5 vials containing lyophilized PMA
(phorbol 12-myristate 13-acetate), 1 vial is intended for
stimulation of 20 positive controls.
Lysing Solution – 15 ml
Heyworth P, Cross A, Curnutte J (2003) Chronic granulomatous disease. Curr Opin Immunol 15(5): 578-84
Winkelstein J, Marino M, Johnston R et al (2000) Chronic
granulomatous disease. Report on a national registry of 368 patients. Medicine (Baltimore) 79 (3):155-69
Sawyer DW, Donowitz GR, Mandell GL (1989)
Polymorphonuclear neutrophils: An effective antimicrobial force. Rev Infect Dis 11: 1532-44
Data obtained with granulocytes of a healthy individual (A), of a chronic granulomatous diease (CGD) male patient (B) and of two females both having one CGD-allele and one non-CGD allele on the X chromosome (C, D). Negative control in blue. Stimulation indexes (SI) depend on particular experimental conditions, but their values are in the rough 10-times lower in case of defected phagocytosis than in case of healthy individuals.