System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
a-z list
Diagnostic Product

BrdUFlowEx® FITC Kit

Product No. Quantity Stock Price Datasheet Shop
ED7073 100 tests yes choose region View datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Description

The BrdUFlowEx® FITC Kit is intended for labeling and subsequent immunodetection of proliferating cells by flow cytometry. The kit can be used to analyze proliferation of human or mouse leukocytes and variety of human and murine cell lines.

The BrdUFlowEx® FITC Kit utilizes unique DNA revealing method known as "atomic scissors". The kit can be used not only for identification of proliferating cells, but also for simultaneous detection of nuclear proteins including those which are usually lost during other DNA revealing methods.

The BrdUFlowEx® FITC Kit is compatible with EdU staining, as the anti-BrdU antibody (clone MoBu-1) does not crossreact with EdU.

Regulatory Status

Format/Formulation

BrdUFlowEx® FITC Kit contains following reagents:

1. ED7073-1
Fix and Lysing Solution: 1 vial containing 10x concentrated fixation and erythrocyte lysing solution, the vial is intended for 100 reactions.

2. ED7073-2
Permeabilizing Solution: 1 vial containing 30 ml of ready-to-use cellular membrane permeabilizing solution, the vial is intended for 100 reactions.

3. ED7073-3
AS Solution 1: (the abbreviation AS stands for atomic scissors), 4 vials containing lyophilized sodium ascorbate, 1 vial is intended for 25 reactions.

4. ED7073-4
AS Solution 2: (the abbreviation AS stands for atomic scissors), 1 vial containing 10 ml of ready-to-use copper (II) solution, the vial is intended for 100 reactions.

5. ED7073-5
AS Solution 3: (the abbreviation AS stands for atomic scissors), 2 vials containing 30 ml of ready-to-use buffer, one vial is intended for 50 reactions.

6. ED7073-6
Anti-BrdU FITC: 1 vial containing 1 ml of ready-to-use solution containing FITC labeled anti-BrdU antibody (clone MoBu-1, IgG1), the vial is intended for 100 reactions.

7. ED7073-7
7-AAD: 1 vial containing 2 ml of ready-to-use 7-aminoactinomycin D solution, the vial is intended for 100 reactions.

8. ED7073-8
BrdU: 4 vials containing lyophilized 5-bromo-2-deoxyuridine, 1 vial is intended to prepare 2 ml of 10 mM BrdU solution.

References

  • Unpublished.
  • Example Data

    Fig.
    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy