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BasoFlowEx® Kit

Product No. Quantity Stock Price Datasheet Shop
ED7043 100 tests yes choose region View datasheet buy
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The BasoFlowEx® Kit is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation. A commercially available allergens (e.g. for prick tests) can be used for the stimulation.

Regulatory Status


BasoFlowEx® Kit contains following reagents:
1. ED7043-1
Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes.
2. ED7043-2
Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls.
3. ED7043-3
Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled.
4. ED7043-4
Lysing Solution – 30 ml.

General references

  • Product Specific References

  • The anti-CD203c antibody was described during HLDA VIII workshop.
    The anti-CD63 antibody MEM-259 was described in: Cerny J et al., EMBO Rep. 2004 Sep;5(9):883-8.
  • *Rentzos G, Lundberg V, Lundqvist C, Rodrigues R, van Odijk J, Lundell AC, Pullerits T, Telemo E: Use of a basophil activation test as a complementary diagnostic tool in the diagnosis of severe peanut allergy in adults. Clin Transl Allergy. 2015 Jun 11;5:22. doi: 10.1186/s13601-015-0064-9. [Abstract] [Full Text]
  • Example Data


    Fig. 1. Delimitation of basophil population (CD203cpos/ SSClow).


    Fig. 2a Histogram of negative control basophils.


    Fig. 2b Histogram of allergen-stimulated basophils.


    Fig. 2c Histogram of positive control basophils.

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