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ApoFlowEx® FITC Kit

Product No. Quantity Stock Price Datasheet Shop
ED7044 100 tests yes choose region View datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order


Apoptosis (a programmed cell death) is a well described phenomenon that occurs in many cellular systems. Currently, the most widely used analytical assays are based on monitoring of translocation of phosphatidylserine (PS) from inner phospholipid layer to the cell surface by use of a fluorochrome-labelled Annexin V in combination with appropriate vital dyes. ApoFlowEx® FITC Kit is based on standard setup that employs Annexin V-FITC conjugate and propidium iodide (PI). The flow cytometry test can discriminate intact cells (FITC-/PI-), apoptotic cells (FITC+/PI-) and necrotic cells (FITC+/PI+).

Regulatory Status


Annexin V-FITC – 1 vial. 0.5 ml of Ready-to-Use solution. No reconstitution is necessary. Reagent is provided in phosphate buffered saline (PBS) containing 15mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Sufficient for 100 tests.
Propidium Iodide – 1 vial. 0.5 ml of Ready-to-Use solution in deionized water (0.1 mg/ml). Sufficient for 100 tests.
Annexin V Binding Buffer (10x) – 1 vial. 5 ml of 10x concentrated filter-sterilized solution (0.1M HEPES/NaOH, pH 7.4, 1.4M NaCl, 25mM CaCl2 ). Dilute 10x in deionized water prior to use.


  • Unpublished.
  • Example Data

    ApoFlowEx FITC Kit

    Fig. 1. Detection of viable, early apoptotic and necrotic / late apoptotic cells in camptothecin-treated cell population using ApoFlowEx® FITC Kit.

    ED7044 FC

    Fig. 2. Flow cytometry staining of apoptotic JURKAT cells with ApoFlowEx® FITC Kit (annexin V FITC and PI).
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