ApoFlowEx® FITC Kit
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Apoptosis (a programmed cell death) is a well described phenomenon that occurs in many cellular systems. Currently, the most widely used analytical assays are based on monitoring of translocation of phosphatidylserine (PS) from inner phospholipid layer to the cell surface by use of a fluorochrome-labelled Annexin V in combination with appropriate vital dyes. ApoFlowEx® FITC Kit is based on standard setup that employs Annexin V-FITC conjugate and propidium iodide (PI). The flow cytometry test can discriminate intact cells (FITC-/PI-), apoptotic cells (FITC+/PI-) and necrotic cells (FITC+/PI+).
– 1 vial. 0.5 ml of Ready-to-Use solution. No reconstitution is necessary.
Reagent is provided in phosphate buffered saline (PBS)
containing 15mM sodium azide and 0.2% (w/v) high-grade
protease free Bovine Serum Albumin (BSA) as a
stabilizing agent. Sufficient for 100 tests.
– 1 vial. 0.5 ml of Ready-to-Use solution in deionized water (0.1 mg/ml). Sufficient
for 100 tests.
Annexin V Binding Buffer (10x)
– 1 vial. 5
ml of 10x concentrated filter-sterilized solution (0.1M
HEPES/NaOH, pH 7.4, 1.4M NaCl, 25mM CaCl2
10x in deionized water prior to use.
Detection of viable, early apoptotic and necrotic / late apoptotic cells in camptothecin-treated cell population using ApoFlowEx® FITC Kit: