EXBIO Antibodies Product Data Sheet


Monoclonal Antibody to HLA-ABCE
Purified Antibody (0.1 mg)

Clone: TP25.99SF
Isotype: Mouse IgG1
Specificity: The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
Regulatory Status: RUO
Immunogen: IFN-gamma-treated human melanoma cells Colo 38
Species Reactivity: Human
Flow Cytometry
Western Blotting
Immunohistochemistry (frozen sections)
Purity: > 95% (by SDS-PAGE)
Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Expiration: See vial label
Lot Number: See vial label
Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
*Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992 May 15;148(10):3202-9.
*Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED: HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61(11):1177-95.
*Blaschitz A, Hutter H, Leitner V, Pilz S, Wintersteiger R, Dohr G, Sedlmayr P: Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Hum Immunol. 2000 Nov;61(11):1074-85.
*Desai SA, Wang X, Noronha EJ, Zhou Q, Rebmann V, Grosse-Wilde H, Moy FJ, Powers R, Ferrone S: Structural relatedness of distinct determinants recognized by monoclonal antibody TP25.99 on beta 2-microglobulin-associated and beta 2-microglobulin-free HLA class I heavy chains. J Immunol. 2000 Sep 15;165(6):3275-83.
*Perosa F, Luccarelli G, Prete M, Favoino E, Ferrone S, Dammacco F: Beta 2-microglobulin-free HLA class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide. J Immunol. 2003 Aug 15;171(4):1918-26.
*Moy FJ, Desai SA, Wang X, Noronha EJ, Zhou Q, Ferrone S, Powers R: Analysis by NMR spectroscopy of the structural homology between the linear and the cyclic peptide recognized by anti-human leukocyte antigen class I monoclonal antibody TP25.99. J Biol Chem. 2000 Aug 11;275(32):24679-85.
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.


For laboratory research only, not for drug, diagnostic or other use.

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