EXBIO Antibodies Product Data Sheet


Monoclonal Antibody to TPX2
Purified Antibody (0.1 mg)

Clone: TPX2-01
Isotype: Mouse IgG1
Specificity: The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
Regulatory Status: RUO
Immunogen: Human TPX2
Species Reactivity: Human, Mouse, Rat
Western Blotting
Immunohistochemistry (paraffin sections)
Purity: > 95% (by SDS-PAGE)
Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage / Stability: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Expiration: See vial label
Lot Number: See vial label
Background: TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.
*Miwa T, Kokuryo T, Yokoyama Y, Yamaguchi J, Nagino M: Therapeutic potential of targeting protein for Xklp2 silencing for pancreatic cancer. Cancer Med. 2015 Jul;4(7):1091-100
*Zhang H, Zhang T, You Z, Zhang Y: Positive Surgical Margin, HPV Persistence, and Expression of Both TPX2 and PD-L1 Are Associated with Persistence/Recurrence of Cervical Intraepithelial Neoplasia after Cervical Conization. PLoS One. 2015 Dec 1;10(12):e0142868.
Wang J, Liu Z, Dou C, Han S, Li C, Tu K, Yang W: miR-491 inhibits the proliferation, invasion and migration of hepatocellular carcinoma cell via down-regulating TPX2 expression. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Apr;32(4):512-7.
*Tomii C, Inokuchi M, Takagi Y, Ishikawa T, Otsuki S, Uetake H, Kojima K, Kawano T: TPX2 expression is associated with poor survival in gastric cancer. World J Surg Oncol. 2017 Jan 9;15(1):14. doi: 10.1186/s12957-016-1095-y
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.


For laboratory research only, not for drug, diagnostic or other use.

EXBIO Praha | Nad Safinou II 341 | 252 50 Vestec u Prahy | Czech Republic

Tel: +420 261 090 666 | Fax: +420 261 090 660 | orders@exbio.cz | www.exbio.cz