EXBIO Antibodies Product Data Sheet

Monoclonal Antibody to CD11b activation epitope
Purified Antibody (0.1 mg)

Clone:	CBRM1/5
Isotype:	Mouse IgG1
Specificity:	The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and does not recognize CD11b on non-activated cells.
Regulatory Status:	RUO
Immunogen:	Information not available
Species Reactivity:	Human
Application:	Flow Cytometry Immunoprecipitation Functional Application blocking
Purity:	> 95% (by SDS-PAGE)
Purification:	Purified by protein-A affinity chromatography
Concentration:	1 mg/ml
Storage Buffer:	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage / Stability:	Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Expiration:	See vial label
Lot Number:	See vial label
Background:	CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
References:	Diamond MS, Springer TA: A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol. 1993 Jan;120(2):545-56. Fagerholm SC, Varis M, Stefanidakis M, Hilden TJ, Gahmberg CG: alpha-Chain phosphorylation of the human leukocyte CD11b/CD18 (Mac-1) integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasation. Blood. 2006 Nov 15;108(10):3379-86.

Clone:

CBRM1/5

Isotype:

Mouse IgG1

Specificity:

The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and does not recognize CD11b on non-activated cells.

Regulatory Status:

RUO

Immunogen:

Information not available

Species Reactivity:

Human

Application:

Flow Cytometry

Immunoprecipitation

Functional Application

blocking

Purity:

> 95% (by SDS-PAGE)

Purification:

Purified by protein-A affinity chromatography

Concentration:

1 mg/ml

Storage Buffer:

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage / Stability:

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Expiration:

See vial label

Lot Number:

See vial label

Background:

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

References:

*Diamond MS, Springer TA: A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol. 1993 Jan;120(2):545-56.

*Fagerholm SC, Varis M, Stefanidakis M, Hilden TJ, Gahmberg CG: alpha-Chain phosphorylation of the human leukocyte CD11b/CD18 (Mac-1) integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasation. Blood. 2006 Nov 15;108(10):3379-86.

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For laboratory research only, not for drug, diagnostic or other use.

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11-172-C100

Monoclonal Antibody to CD11b activation epitope Purified Antibody (0.1 mg)

Monoclonal Antibody to CD11b activation epitope
Purified Antibody (0.1 mg)