EXBIO Antibodies Product Data Sheet


Monoclonal Antibody to CD137 / 4-1BB
Low Endotoxin (0.1 mg)

Clone: 4B4-1
Isotype: Mouse IgG1
Specificity: The mouse monoclonal antibody 4B4-1 recognizes CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.
HLDA VI; WS Code C-7
Regulatory Status: RUO
Immunogen: Recombinant human CD137 ectodomain
Species Reactivity: Human, Non-Human Primates
Flow Cytometry
Functional Application
inhibition of cytokine production
Purity: > 95% (by SDS-PAGE)
Purification: Purified by protein-A affinity chromatography
Concentration: 1 mg/ml
Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage / Stability: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Expiration: See vial label
Lot Number: See vial label
Background: CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.
*Chan SL, Voskens CJ, Lin W, Schindler DG, Azimzadeh A, Wang LX, Taylor RJ, Strome SE, Schulze DH: Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non-human primate models. J Mol Recognit. 2009 May-Jun;22(3):242-9. doi: 10.1002/jmr.937.
*Yi L, Zhao Y, Wang X, Dai M, Hellström KE, Hellström I, Zhang H: Human and mouse CD137 have predominantly different binding CRDs to their respective ligands. PLoS One. 2014 Jan 21;9(1):e86337.
*Fernández Do Porto DA, Jurado JO, Pasquinelli V, Alvarez IB, Aspera RH, Musella RM, García VE: CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis. Immunol Cell Biol. 2012 Apr;90(4):449-56.
*Bellarosa D, Bressan A, Bigioni M, Parlani M, Maggi CA, Binaschi M: SAHA/Vorinostat induces the expression of the CD137 receptor/ligand system and enhances apoptosis mediated by soluble CD137 receptor in a human breast cancer cell line. Int J Oncol. 2012 Oct;41(4):1486-94.
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For laboratory research only, not for drug, diagnostic or other use.

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