EXBIO Antibodies Product Data Sheet

11-737-C025

Monoclonal Antibody to CD3 zeta (Phospho-Tyr111)
Purified Antibody (0.025 mg)

Clone: EM-55
Isotype: Mouse IgG1
Specificity: The mouse monoclonal antibody EM-55 recognizes phosphorylated tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Regulatory Status: RUO
Immunogen: BP1 (pTyr111/123) - KLH
Species Reactivity: Human, Mouse
Application:
Flow Cytometry
Intracellular staining
Western Blotting
Purity: > 95% (by SDS-PAGE)
Purification: Purified by protein-A affinity chromatography
Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage / Stability: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Expiration: See vial label
Lot Number: See vial label
Background: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
References: Unpublished.
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.

 


For laboratory research only, not for drug, diagnostic or other use.

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