|Specificity:||The mouse monoclonal antibody E11 recognizes CD35 (CR1), a type I glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
HLDA III; WS Code 204
|Immunogen:||Acute monocytic leukemia cells and normal blood monocytes|
|Species Reactivity:||Human, Non-Human Primates|
Immunohistochemistry (paraffin sections)
Application note:heat mediated antigen retrieval
Immunohistochemistry (frozen sections)
Application note:acetone fixation
|Purity:||> 95% (by SDS-PAGE)|
|Purification:||Purified by protein-A affinity chromatography|
|Storage Buffer:||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage / Stability:||Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.|
|Expiration:||See vial label|
|Lot Number:||See vial label|
|Background:||CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.|
*Kremlitzka M, Polgár A, Fülöp L, Kiss E, Poór G, Erdei A: Complement receptor type 1 (CR1, CD35) is a potent inhibitor of B-cell functions in rheumatoid arthritis patients. Int Immunol. 2012 Sep 7. [Epub ahead of print]
*Nielsen CH, Pedersen ML, Marquart HV, Prodinger WM, Leslie RG: The role of complement receptors type 1 (CR1, CD35) and 2 (CR2, CD21) in promoting C3 fragment deposition and membrane attack complex formation on normal peripheral human B cells. Eur J Immunol. 2002 May;32(5):1359-67.
*Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611.
*Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ: Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236-43.
For laboratory research only, not for drug, diagnostic or other use.
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