EXBIO Antibodies Product Data Sheet

11-703-C100

Monoclonal Antibody to CD35
Purified Antibody (0.1 mg)

Clone: E11
Isotype: Mouse IgG1
Specificity: The mouse monoclonal antibody E11 recognizes CD35 (CR1), a type I glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
HLDA III; WS Code 204
Regulatory Status: RUO
Immunogen: Acute monocytic leukemia cells and normal blood monocytes
Species Reactivity: Human, Non-Human Primates
Application:
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunohistochemistry (paraffin sections)
Application note:heat mediated antigen retrieval
Immunohistochemistry (frozen sections)
Application note:acetone fixation
Immunocytochemistry
Purity: > 95% (by SDS-PAGE)
Purification: Purified by protein-A affinity chromatography
Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage / Stability: Store at 2-8C. Do not freeze. Do not use after expiration date stamped on vial label.
Expiration: See vial label
Lot Number: See vial label
Background: CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.
References:
*Kremlitzka M, Polgr A, Flp L, Kiss E, Por G, Erdei A: Complement receptor type 1 (CR1, CD35) is a potent inhibitor of B-cell functions in rheumatoid arthritis patients. Int Immunol. 2012 Sep 7. [Epub ahead of print]
*Nielsen CH, Pedersen ML, Marquart HV, Prodinger WM, Leslie RG: The role of complement receptors type 1 (CR1, CD35) and 2 (CR2, CD21) in promoting C3 fragment deposition and membrane attack complex formation on normal peripheral human B cells. Eur J Immunol. 2002 May;32(5):1359-67.
*Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611.
*Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ: Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236-43.
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIOs term and conditions which are available at www.exbio.cz.

 


For laboratory research only, not for drug, diagnostic or other use.

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