|Specificity:||The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).|
|Immunogen:||High Mw glycoproteins from rat thymocytes|
Immunohistochemistry (paraffin sections)
Immunohistochemistry (frozen sections)
|Purity:||> 95% (by SDS-PAGE)|
|Purification:||Purified from cell culture supernatant by protein-A affinity chromatography.|
|Storage Buffer:||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage / Stability:||Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.|
|Expiration:||See vial label|
|Lot Number:||See vial label|
|Background:||The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.|
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For laboratory research only, not for drug, diagnostic or other use.
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