| Clone: | UW60.1 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The mouse monoclonal antibody UW60.1 recognizes human CD209 (DC-SIGN), a 44 kDa transmembrane receptor expressed on the surface of dendritic cells and macrophages. |
| Immunogen: | CD209-His-tagged fusion protein |
| Species Reactivity: | Human |
| Application: |
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | CD209, also known as DC-SIGN (dendritic cell-specific ICAM-3-grabbing nonintegrin) is a transmembrane receptor expressed on the surface of dendritic cells and macrophages, which recognizes numerous pathogens ranging from parasites to viruses. Its N-terminal domain is transmembrane, whereas a tandem-repeat neck domain and the C terminal C-type lectin carbohydrate recognition domain have dual function as a pathogen recognition receptor and a cell adhesion receptor. The neck region is responsible for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. A ligand of CD209 is also CD50 (ICAM-3). |
| References: |
*Khoo US, Chan KY, Chan VS, Lin CL: DC-SIGN and L-SIGN: the SIGNs for infection. J Mol Med. 2008 Aug;86(8):861-74.
*van Kooyk Y, Geijtenbeek TB: A novel adhesion pathway that regulates dendritic cell trafficking and T cell interactions. Immunol Rev. 2002 Aug;186:47-56.
*Geijtenbeek TB, Engering A, Van Kooyk Y: DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 2002 Jun;71(6):921-31.
*Ryan EJ, Marshall AJ, Magaletti D, Floyd H, Draves KE, Olson NE, Clark EA: Dendritic cell-associated lectin-1: a novel dendritic cell-associated, C-type lectin-like molecule enhances T cell secretion of IL-4. J Immunol. 2002 Nov 15;169(10):5638-48.
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