| Clone: | V65 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The mouse monoclonal antibody V65 recognizes TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection. |
| Immunogen: | TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line |
| Species Reactivity: | Rat |
| Preparation: | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Concentration: | 0.5 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. |
| Usage: | The reagent is designed for Flow Cytometry analysis. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression. |
| References: |
*Giezeman-Smits KM, Jonges LE, Chambers WH, Brisette-Storkus CS, Van Vlierberghe RL, Van Eendenburg JD, Eggermont AM, Fleuren GJ, Kuppen PJ: Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells. J Leukoc Biol. 1998 Feb;63(2):209-15.
*Hosea HJ, Rector ES, Taylor CG: Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in
spleen and blood. J Nutr. 2003 Dec;133(12):4239-42.
*Tam FW, Smith J, Morel D, Karkar AM, Thompson EM, Cook HT, Pusey CD: Development of scarring and renal failure in a rat model of crescentic glomerulonephritis. Nephrol Dial Transplant. 1999 Jul;14(7):1658-66.
*Henriksson G, Helgeland L, Midtvedt T, Stierna P, Brandtzaeg P: Immune response to Mycoplasma pulmonis in nasal mucosa is modulated by the normal microbiota. Am J Respir Cell Mol Biol. 2004 Dec;31(6):657-62.
Xystrakis E, Dejean AS, Bernard I, Druet P, Liblau R, Gonzalez-Dunia D, Saoudi A: Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation. Blood. 2004 Nov 15;104(10):3294-301.
*Moghaddami M, Cleland LG, Mayrhofer G: MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype,
comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro. Int Immunol. 2005 Aug;17(8):1103-15.
*van den Brandt J, Kwon SH, Hünig T, McPherson KG, Reichardt HM: Sustained pre-TCR expression in Notch1IC-transgenic rats impairs T cell maturation and selection. J Immunol. 2005 Jun 15;174(12):7845-52.
*De Bernardis F, Lucciarini R, Boccanera M, Amantini C, Arancia S, Morrone S, Mosca M, Cassone A, Santoni G: Phenotypic and functional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis. Infect Immun. 2006 Jul;74(7):4282-94.
*And many other.
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