| Clone: | Polyclonal |
|---|---|
| Isotype: | Rabbit None |
| Specificity: | The rabbit polyclonal antibody recognizes C-terminus of metabotropic glutamate receptor 1 isoform mGluR1b. The mGluR1a isoform is not recognized. |
| Immunogen: | KLH-coupled synthetic peptide derived from the last 19 amino acids of rat/human mGluR1b. Only 1 amino acid is different from the mouse last 19 amino acids of mGluR1b (V-->A). |
| Species Reactivity: | Mouse, Other not tested |
| Application: |
Western Blotting
Recommended dilution:5 μg/ml
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from rabbit serum by protein-G affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | The mGluR1b protein is an isoform of the metabotropic glutamate receptor 1 (mGluR1). The mGluR1, together with mGluR5 (both constituting group I of mGluRs), is coupled to the inositol phosphate/Ca[2+] signal transduction pathway, whereas the other five metabotropic glutamate receptors (mGluR2, mGluR3, mGluR4, mGluR6, mGluR7 ) are linked to the inhibition of the cAMP cascade. The highest level of mGluR1 mRNA expression is found in the cerebellar Purkinje cells. Three splicing variants have been described for mGluR1: mGluR1a (alpha), mGluR1b (beta), and mGluR1c. The mGluR1a and mGluR1b functionally differ e.g. in their internalisation responses or in ability to activate the Gs protein pathway, as the mGluR1b lacks a part of C terminus. |
| References: |
*Song JH, Park ES, Han SM, Han SR, Ahn DK, Youn DH: Signal transduction mechanisms underlying group I mGluR-mediated increase in frequency and amplitude of spontaneous EPSCs in the spinal trigeminal subnucleus
oralis of the rat. Mol Pain. 2009 Sep 2;5:50.
*Ciruela F, McIlhinney RA: Differential internalisation of mGluR1 splice variants in response to agonist and
phorbol esters in permanently transfected BHK cells. FEBS Lett. 1997 Nov 24;418(1-2):83-6.
*Tateyama M, Kubo Y: Coupling profile of the metabotropic glutamate receptor 1alpha is regulated by the C-terminal domain. Mol Cell Neurosci. 2007 Mar;34(3):445-52.
*Hong YH, Kim JY, Lee JH, Chae HG, Jang SS, Jeon JH, Kim CH, Kim J, Kim SJ: Agonist-induced internalization of mGluR1alpha is mediated by caveolin. J Neurochem. 2009 Oct;111(1):61-71.
*Marcaggi P, Mutoh H, Dimitrov D, Beato M, Knöpfel T: Optical measurement of mGluR1 conformational changes reveals fast activation, slow deactivation, and sensitization. Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11388-93.
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