|Specificity:||The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.|
|Immunogen:||SDS extracted protein fraction of human myocardium|
|Species Reactivity:||Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken|
Recommended dilution:1 μg/ml
Positive control:murine femoral muscle, murine heart
Negative control:HUVEC line
Application note:reducing conditions
Immunohistochemistry (paraffin sections)
Application note:Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
Immunohistochemistry (frozen sections)
|Purity:||> 95% (by SDS-PAGE)|
|Purification:||Purified from cell culture supernatant by protein-A affinity chromatography.|
|Storage Buffer:||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage / Stability:||Store at 2-8░C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20░C. Avoid freeze/thaw cycles.|
|Expiration:||See vial label|
|Lot Number:||See vial label|
|Background:||Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.|
*Tsukada T, Tippens D, Gordon D, Ross R, Gown AM: HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol. 1987 Jan;126(1):51-60.
*Tsukada T, McNutt MA, Ross R, Gown AM: HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues. Am J Pathol. 1987 May;127(2):389-402.
*Schmidt RA, Cone R, Haas JE, Gown AM: Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins. Am J Pathol. 1988 Apr;131(1):19-28.
*Miettinen M: Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors. Am J Pathol. 1988 Jan;130(1):205-15.
*de Sousa SO, Schwarzschild M, de Ara˙jo NS, de Ara˙jo VC: Basal cell adenocarcinoma of the palate with squamous metaplasia. J Clin Pathol. 2000 Feb;53(2):153-6.
*Handharyani E, Ochiai K, Iwata N, Umemura T: Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens. J Vet Med Sci. 2001 May;63(5):547-52.
*Ijzer J, Roskams T, Molenbeek RF, Ultee T, Penning LC, Rothuizen J, van den Ingh TS: Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver. Comp Hepatol. 2006 Nov 16;5:7.
*Matsuyama A, Hisaoka M, Hashimoto H: Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma. Hum Pathol. 2007 Apr;38(4):645-51.
*Matsuo S, Susumu S, Tsutsumi R, Azuma T, Obata S, Hayashi T: Glomus tumor of the omentum: a case report. J Surg Oncol. 2007 Dec 1;96(7):633-6.
*And many other.
For laboratory research only, not for drug, diagnostic or other use.
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