EXBIO Antibodies Product Data Sheet

Monoclonal Antibody to CD117
Allophycocyanin (APC) conjugated (100 tests)

Clone:	104D2
Isotype:	Mouse IgG1
Specificity:	The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen. HLDA VI; WS Code C-30
Immunogen:	MOLM-1 megakaryocytic cells
Species Reactivity:	Human, Non-Human Primates, Bovine
Preparation:	The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage Buffer:	The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Storage / Stability:	Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Usage:	The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Expiration:	See vial label
Lot Number:	See vial label
Background:	CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.
References:	Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997 Jul 1;90(1):111-25. Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998 Feb 1;91(3):898-906. Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c-Kit. Blood. 1999 Sep 15;94(6):1979-86. Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49(2):97-110. Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117). Exp Hematol. 2000 Jun;28(6):660-71. Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk Res. 2006 Oct;30(10):1241-8. Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1;110(1):151-60. Stevenson KS, Mc Glynn L, Hodge M, Mc Linden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem Cells Dev. 2009 Mar 27. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). And other.

Clone:

104D2

Isotype:

Mouse IgG1

Specificity:

The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
HLDA VI; WS Code C-30

Immunogen:

MOLM-1 megakaryocytic cells

Species Reactivity:

Human, Non-Human Primates, Bovine

Preparation:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage Buffer:

The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage / Stability:

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Usage:

The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
The content of a vial (1 ml) is sufficient for 100 tests.

Expiration:

See vial label

Lot Number:

See vial label

Background:

CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.

References:

*Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997 Jul 1;90(1):111-25.

*Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998 Feb 1;91(3):898-906.

*Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c-Kit. Blood. 1999 Sep 15;94(6):1979-86.

*Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49(2):97-110.

*Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117). Exp Hematol. 2000 Jun;28(6):660-71.

*Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk Res. 2006 Oct;30(10):1241-8.

*Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1;110(1):151-60.

*Stevenson KS, Mc Glynn L, Hodge M, Mc Linden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem Cells Dev. 2009 Mar 27.

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*And other.

For laboratory research only, not for drug, diagnostic or other use.

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Tel: +420 261 090 666 | Fax: +420 261 090 660 | orders@exbio.cz | www.exbio.cz

1A-586-T100

Monoclonal Antibody to CD117 Allophycocyanin (APC) conjugated (100 tests)

Monoclonal Antibody to CD117
Allophycocyanin (APC) conjugated (100 tests)