| Clone: | AGR3.4 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis. |
| Immunogen: | Purified human AGR3 protein |
| Species Reactivity: | Human, Other species Not tested |
| Application: |
Western Blotting
Recommended dilution: 1 μg/ml
Positive control: T47D breast cancer cell line Negative control: H1299 lung carcinoma cell line
Immunohistochemistry (paraffin sections)
Recommended dilution:5 μg/ml
Positive tissue:human colon
Immunocytochemistry
Recommended dilution: 1 μg/ml
Positive control: T47D breast cancer cell line Negative control: H1299 lung carcinoma cell line |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from ascites by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development. |
| References: |
*Persson S, Rosenquist M, Knoblach B, Khosravi-Far R, Sommarin M, Michalak M: Diversity of the protein disulfide isomerase family: identification of breast tumor induced Hag2 and Hag3 as novel members of the protein family. Mol Phylogenet Evol. 2005 Sep;36(3):734-40.
*Fletcher GC, Patel S, Tyson K, Adam PJ, Schenker M, Loader JA, Daviet L, Legrain P, Parekh R, Harris AL, Terrett JA: hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with
metastasis gene C4.4a and dystroglycan. Br J Cancer. 2003 Feb 24;88(4):579-85.
*Zhang JS, Gong A, Cheville JC, Smith DI, Young CY: AGR2, an androgen-inducible secretory protein overexpressed in prostate cancer. Genes Chromosomes Cancer. 2005 Jul;43(3):249-59.
*Zweitzig DR, Smirnov DA, Connelly MC, Terstappen LW, O'Hara SM, Moran E: Physiological stress induces the metastasis marker AGR2 in breast cancer cells. Mol Cell Biochem. 2007 Dec;306(1-2):255-60.
*Ramachandran V, Arumugam T, Wang H, Logsdon CD: Anterior gradient 2 is expressed and secreted during the development of
pancreatic cancer and promotes cancer cell survival. Cancer Res. 2008 Oct 1;68(19):7811-8.
|
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