| Clone: | EM-22 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody EM-22 reacts with C-terminal part of beta-catenin, an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription. |
| Immunogen: | Recombinant human beta-catenin |
| Species Reactivity: | Human, Mouse, Hamster |
| Application: |
Flow Cytometry
Immunoprecipitation
Western Blotting
Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line
KW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line
Immunocytochemistry
Positive control: HT29 human colon adenocarcinoma cell line
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted. |
| References: |
*Nowak M, Madej JA, Dziegiel P.: Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia Histochem Cytobiol. 2007;45(3):233-8.
*Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS.: Epstein-Barr Virus, Beta-Catenin, and E-cadherin in Gastric Carcinomas. J Korean Med Sci. 2007 Oct;22(5):855-61.
*Valenta T, Lukas J, Korinek V.: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31(9):2369-80.
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