| Clone: | SOS-01 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein. |
| Immunogen: | Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein. |
| Species Reactivity: | Human, Mouse, Other not determined |
| Application: |
Western Blotting
Recommended dilution: 1 μg/ml
Positive control: HeLa human cervix carcinoma cell line Application note: Reducing conditions.
Immunocytochemistry
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from ascites by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex. |
| References: |
*Tian X, Feig LA: Basis for signaling specificity difference between Sos and Ras-GRF guanine nucleotide exchange factors. J Biol Chem. 2001 Dec 14;276(50):47248-56.
*Innocenti M, Tenca P, Frittoli E, Faretta M, Tocchetti A, Di Fiore PP, Scita G.: Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002 Jan 7;156(1):125-36.
*Silver SJ, Chen F, Doyon L, Zink AW, Rebay I: New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function. Genesis. 2004 Aug;39(4):263-72.
*Freedman TS, Sondermann H, Friedland GD, Kortemme T, Bar-Sagi D, Marqusee S, Kuriyan J.: A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16692-7.
*Yang L, Bashaw G.J.: Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline. Neuron. 2006 Nov 22;52(4):595-607.
|
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