| Clone: | ARAP1-2 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein. |
| Immunogen: | His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1 |
| Species Reactivity: | Human |
| Application: |
Immunoprecipitation
Western Blotting
Recommended dilution: 2 μg/ml
Immunocytochemistry
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from ascites by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading. |
| References: |
*Miura K, Jacques KM, Stauffer S, Kubosaki A, Zhu K, Hirsch DS, Resau J, Zheng Y, Randazzo PA: ARAP1: a point of convergence for Arf and Rho signaling. Mol Cell. 2002 Jan;9(1):109-19.
*Guo DF, Chenier I, Tardif V, Orlov SN, Inagami T: Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. Biochem Biophys Res Commun. 2003 Oct 31;310(4):1254-65.
*Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
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