EXBIO Antibodies Product Data Sheet

10-502-C025

Monoclonal Antibody to CD11b
Azide Free (0.025 mg)

Clone: MEM-170
Isotype: Mouse IgG1
Specificity: The antibody MEM-170 recognizes CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
HLDA VI., WS Code A054
Regulatory Status: RUO
Immunogen: Human granulocytes
Species Reactivity: Human
Application:
Flow Cytometry
Recommended dilution:1 μg/ml
Functional Application
The antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
Purity: > 95% (by SDS-PAGE)
Purification: Purified by protein-A affinity chromatography
Concentration: 1 mg/ml
Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized.
Storage / Stability: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Expiration: See vial label
Lot Number: See vial label
Background: CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
References:
*Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20.
*Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E.: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606.
*Lawrence PK, Srikumaran S.: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98.
*Leukocyte Typing VI. Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.

 


For laboratory research only, not for drug, diagnostic or other use.

EXBIO Praha | Nad Safinou II 341 | 252 50 Vestec u Prahy | Czech Republic

Tel: +420 261 090 666 | Fax: +420 261 090 660 | orders@exbio.cz | www.exbio.cz