| Clone: | S-tag-05 |
|---|---|
| Isotype: | Mouse IgG2b |
| Specificity: | The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. |
| Immunogen: | GST fusion protein |
| Species Reactivity: | Recognizes fusion proteins in all species |
| Application: |
Western Blotting
Recommended dilution: 1 μg/ml
Positive control: GST fusion protein Application note: Reducing conditions
Immunoprecipitation
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins. |
| References: | Unpublished. |
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