| Clone: | MT-07 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody MT-07 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab). |
| Immunogen: | Microtubule protein (bovine brain) enriched for kinesin |
| Species Reactivity: | Human, Porcine, Mouse |
| Application: |
Immunoprecipitation
Positive material: Porcine brain
Western Blotting
Positive control:
Porcine brain Microtubule associated protein 2 (MAP2)
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: brain
Immunohistochemistry (frozen sections)
Positive tissue: Mouse brain
Immunocytochemistry
Positive control: human neuroblastoma SH-SY5Y
ELISA
Positive control:
Porcine brain Microtubule associated protein 2 (MAP2) |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells. |
| References: | Unpublished. |
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