| Clone: | TU-11 |
|---|---|
| Isotype: | Mouse IgM |
| Specificity: | The antibody TU-11 recognizes an epitope located on C-terminal domain of beta-tubulin in various species. |
| Immunogen: | microtubule proteins from porcine brain |
| Species Reactivity: | Porcine, Mouse |
| Application: |
Western Blotting
Recommended dilution: 2 μg/ml, 60 min in room temperature
Positive control: Porcine brain lysate Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 3 min in water bath. Application note: Reducing conditions. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from ascites by precipitation methods and size-exclusion chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. Do not freeze. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. |
| References: |
*Dráber P, Dráberová E, Linhartová I, Viklický V.: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28.
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