EXBIO Antibodies Product Data Sheet

1P-451-T025

Monoclonal Antibody to CD36
Phycoerythrin (PE) conjugated (25 tests)

Clone: TR9
Isotype: Mouse IgG1
Specificity: The antibody TR9 reacts with CD36 (GPIIIb), a 85 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. The antibody TR9 cross-blocks binding of FITC-labeled standard antibody OKM5.
Anti-CD36 antibodies inhibit adhesive functions (e.g. adherence of infected erythrocytes to target cells).
Regulatory Status: RUO
Immunogen: Platelets
Species Reactivity: Human
Preparation: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage Buffer: The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
Storage / Stability: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Usage: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
The content of a vial (0.5 ml) is sufficient for 25 tests.
Expiration: See vial label
Lot Number: See vial label
Background: CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
References:
*Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P: The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse. FASEB J. 2007 Dec 27
*van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin-induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. Biochim Biophys Acta. 2007 Dec 15
*Won WJ, Bachmann MF, Kearney JF: CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen. J Immunol. 2008 Jan 1;180(1):230-7.
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For laboratory research only, not for drug, diagnostic or other use.

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