| Clone: | 01G |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. |
| Immunogen: | HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
| Species Reactivity: | Human |
| Negative Species: | Mouse, Rat |
| Preparation: | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Usage: | The reagent is designed for Flow Cytometry analysis and Immunocytochemistry of cells expressing HLA-G molecule on the cell surface, and ELISA.
Suggested working dilution for Flow Cytometry is 1:200. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| References: |
*Ishitani A. et al., Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Roles for HLA-E, F and G in Maternal-Placental Immune Recognitions. The Journal of Immunology 171, 1376 (2003).
*Real LM, Cabrera T, Collado A, Jimenez P, Garcia A, Ruiz-Cabello F, Garrido F: Expression of HLA G in human tumors is not a frequent event. Int J Cancer. 1999 May 17;81(4):512-8.
*Real LM, Cabrera T, Canton J, Oliva R, Ruiz-Cabello F, Garrido F: Looking for HLA-G expression in human tumours.
J Reprod Immunol. 1999 Jul;43(2):263-73.
*Polakova K, Russ G: Expression of the non-classical HLA-G antigen in tumor cell lines is extremely restricted. Neoplasma. 2000;47(6):342-8.
*Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003;50(5):331-8.
*Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA-G expression in malignant hematopoetic cells from leukemia
patients. Leuk Res. 2003 Jul;27(7):643-8.
*Wiendl H, Feger U, Mittelbronn M, Jack C, Schreiner B, Stadelmann C, Antel J, Brueck W, Meyermann R, Bar-Or A, Kieseier BC, Weller M: Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity. Brain. 2005 Nov;128(Pt 11):2689-2704.
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